Flag antibody cst
WebAnti-FLAG Antibody Products from Cell Signaling Technology The following lists a number of FLAG antibodies from various suppliers. The target antigen refers to the FLAG epitope tag, which is an octapeptide … Web66008-3-Ig targets DYKDDDDK tag in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with recombinant protein samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Flag antibody cst
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WebApr 12, 2024 · We first developed a novel anti-FAP antibody using phage display technology. The binding specificity of the anti-FAP antibody was tested on 3T3-mFAP (Flag) and HT1080-huFAP. The results in Fig. 3a indicated that 8E3-scFv bound specifically to FAP-expressing cells but not to cells without FAP expression. WebApr 11, 2024 · PRRSV anti-N-protein monoclonal antibodies (SDOW17, Korea), anti-HA antibodies (3724 S), and anti-PCNA antibodies (13110) were obtained from Cell Signaling Technology (MA, USA), and anti-PCNA antibodies (AF1363), anti-FLAG antibodies (AF519), and anti-GST antibodies (AF0174) were obtained from Beyotime …
WebMonoclonal Antibody for studying Flag-Tag. Cited in 2 publications. Validated for Immunoprecipitation. Highly specific and rigorously validated in-house, DYKDDDDK Tag (D6W5B) Rabbit Monoclonal Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Sepharose® Bead Conjugate) (CST #70569) is ready to ship. WebDear all, I have a Flag tagged transgenic Drosophila line that I have generated;however, I have specificity issue in the immunofluorescent experiments with CST rabbit polyclonal anti-flag antibody.
WebSpecificity / Sensitivity. K48-linkage Specific Polyubiquitin Antibody detects polyubiquitin chains formed by Lys48 residue linkage. Antibody demonstrates slight cross-reactivity with linear polyubiquitin chain. No cross-reactivity observed with monoubiquitin or polyubiquitin chains formed by specific linkage to different lysine residues. WebApr 12, 2024 · The coverslips were incubated with primary antibodies against USP1 (Cell Signaling Technology, Cat# 8033, 1:200) and TAZ (Abcam, ab224239, 1:200) at 4 °C overnight.
Web2 days ago · Primary antibodies used in this study were as follows: anti-MYC (9B11, Cell Signaling, mouse monoclonal), anti-Flag (F1804, Sigma-Aldrich, mouse monoclonal); secondary antibodies used were Alexa ...
WebWB. Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using MLKL (D2I6N) Rabbit mAb #14993. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the … cu denver business advisingWebMonoclonal Antibody for studying Flag-Tag. Cited in 855 publications. Validated for WB, IP, IHC, IF, F, ChIP. Highly specific and rigorously … cu dental school emergency clinicWebThis product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, … cu denver business school academic advisingWebImmunohistochemical analysis of paraffin-embedded mouse small intestine using Tom20 (D8T4N) Rabbit mAb. Confocal immunofluorescent analysis of HeLa (high expression, left) and Saos-2 (low expression, right) cells, using Tom20 (D8T4N) Rabbit mAb (green). Actin filaments were labeled with DyLight ™ 554 Phalloidin #13054 (red). cu denver cyber security clubWebMonoclonal ANTI-FLAG ® M2 antibody produced in mouse. clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide) View Price and Availability. Sigma-Aldrich. SAB4200071. easter latin americaWebMar 22, 2024 · The protein levels of EGFR, FLAG and β-actin in whole cell lysate (WCL) and Biotin fractions were detected with immunoblotting. The data showed two independent experiments (1st and 2nd). b HEK293T cells were transfected with FLAG-DRAM1 plasmid for 24 h, the cells were fixed and stained with FLAG antibody (red). Representative … easter latinWebApr 14, 2024 · Secondary antibodies (Mouse IgG HRP Linked Whole Ab, NA931, Cytiva, Tokyo, Japan or Anti-rabbit IgG, HRP-linked Antibody #7074, Cell Signaling), were used at a dilution of 1:2000 for 1 h at room ... cu denver christy ridd